Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.
Mouse Lung Mesenchymal StemCells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1:3 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.
Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry.
Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
| Weight | 0.5 kg |
|---|---|
| Dimensions | 10 × 2 × 2 cm |
| Application | For research use only |
| Biosafety Level | 1 |
| Cat.No | ABC-TC3213 |
| Product Type | Mouse Primary Cells |
| Species | Mouse |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Storage | Liquid Nitrogen |
| Disease | Normal |
| Source Organ | Lung |
| Cell Type | Mesenchymal Stem Cell |
| Medium Supplement | Complete Murine Cell Culture Medium |
| Citation Guide | please cite our product as ??AcceGen Biotech Cat.# XXX-0000??. In return, we??ll give you a $100 coupon. Simply click here and submit your paper??s PubMed ID (PMID)., When you publish your research |
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