C57BL/6 Mouse Mammary Fibroblasts


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C57BL/6 Mouse Primary Mammary Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Mammary Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.
Mouse Primary Mammary Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Weight 0.5 kg
Dimensions 10 × 2 × 2 cm

For research use only

Biosafety Level




Product Type

Mouse Primary Cells




1 vial

Shipping Info

Dry Ice


Liquid Nitrogen



Source Organ

Mammary Gland

Cell Type


Medium Supplement

Complete Murine Fibroblast Medium

Citation Guide

please cite our product as ??AcceGen Biotech Cat.# XXX-0000??. In return, we??ll give you a $100 coupon. Simply click here and submit your paper??s PubMed ID (PMID)., When you publish your research


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